Recombinant protein expression by targeting pre-selected chromosomal loci

Recombinant protein expression by targeting pre-selected chromosomal loci

BACKGROUND Recombinant protein expression in mammalian cells is mostly achieved by stable integration of transgenes into the chromosomal DNA of established cell lines. The chromosomal surroundings have strong influences on the expression of transgenes. The exploitation of defined loci by targeting expression constructs with different regulatory elements is an approach to design high level expre...

Towards rational engineering of cells: Recombinant gene expression in defined chromosomal loci

The strength of recombinant gene expression is a key property of cell lines for biopharmaceutical protein production. In most stable cell lines the expression vector is stably introduced into the host chromosomal DNA. Apart from the copy number and the used expression control elements the performance of recombinant expression vectors is modulated by genetic and epigenetic features provided by f...

Advances and challenges in recombinant protein expression in Escherichia coli

Expression of Recombinant Protein B Subunit Pili from Vibrio Cholera

Background & Aims: Vibrio cholerae is a gram-negative bacterial pathogen that causes cholera disease. Following ingestion by a host and entry into the upper intestine, V. cholera colonizes and begins to emit enterotoxin. One of the most pathogenic factors of Vibrio cholera is toxin-coregulated pili (TCP). ToxinCoregulated pili is as the primary factor requiered for the colonization and insisten...

Recombinant protein expression and purification

Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of recombinant DNA technology in order to evaluate any protein of interest, without prior knowledge of the protein’s cellular location or function. The parallel use of affinity tags with recombinant DNA techniques, allows the facile modification of proteins of interest leading to efficient identification, produ...